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Duplex Proximity Sequencing (Pro-Seq): A method to improve DNA sequencing accuracy without the cost of molecular barcoding redundancy

Fig 5

Comparative sequencing performance between amplicon sequencing with high fidelity polymerase (top) and Pro-Seq (bottom).

A wild-type plasma sample was sequenced using a 19-amplicon panel with both methods, and the FPR plotted per base position. Amplicon sequencing (grey) has an average FPR of 1.2 x 10−4 errors per base, compared to Pro-Seq (green), which had an average FPR of 1.1 x 10−6 errors per base (a known SNP at panel position 237 is ignored for FPR calculations).

Fig 5