Novel coronavirus-like particles targeting cells lining the respiratory tract
HF cells were infected (MOI = 1) with E or M or S or control rBVs. Cells were harvested at different times post infection and resolved by 16% or 12% or 8% SDS-PAGE for the E, the M and the S proteins respectively. For E protein visualization gel was stained with Coomassie (a), whereas M and S protein were visualized by Western blot with anti-M (b) and anti-S antibodies (c), respectively. Inactivated HCoV-NL63 was used as positive control for the anti-M antibody.