Characterization of diverse homoserine lactone synthases in Escherichia coli
(A) The modular Sender vector allows facile cloning of any EcoRI, XbaI-flanked synthase open reading frame (ORF) and co-expression with mCherry (mCh) from a single bicistronic mRNA. The vector is represented as SBOL  glyphs (top) and a scaled circular map (bottom). (B) Optical density (OD) readings for E. coli cultures expressing each of the Sender plasmids. (C) mCherry expression is shown as end-point RFP signal normalized to OD600 after an 8 hour growth period.