Oral edible plant vaccine containing hypoallergen of American cockroach major allergen Per a 2 prevents roach-allergic asthma in a murine model
(A) Two different sizes of DNA fragments, one containing the full-length of Per a 2 gene, named 996, and the other containing the central region of Per a 2 gene, named 372, were replaced into the GFP gene in TuMV vector. (B) Plasmids of TuMV-Per a 2–996 and 372 were inoculated into C. quinoa. Symptoms were observed 7–8 days post-inoculation (dpi). Then the local lesion on C. quinoa were used as a viral source to inoculate into Brassica chinensis L. Plants inoculated by TuMV-Per a 2–996 and TuMV-Per a 2–372 showed obvious symptoms at 40 dpi.