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Oral edible plant vaccine containing hypoallergen of American cockroach major allergen Per a 2 prevents roach-allergic asthma in a murine model

Fig 2

The construction of the Per a 2 gene in Turnip mosaic virus (TuMV) vector.

(A) Two different sizes of DNA fragments, one containing the full-length of Per a 2 gene, named 996, and the other containing the central region of Per a 2 gene, named 372, were replaced into the GFP gene in TuMV vector. (B) Plasmids of TuMV-Per a 2–996 and 372 were inoculated into C. quinoa. Symptoms were observed 7–8 days post-inoculation (dpi). Then the local lesion on C. quinoa were used as a viral source to inoculate into Brassica chinensis L. Plants inoculated by TuMV-Per a 2–996 and TuMV-Per a 2–372 showed obvious symptoms at 40 dpi.

Fig 2

doi: https://doi.org/10.1371/journal.pone.0201281.g002