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Identification and characterisation of the CD40-ligand of Sigmodon hispidus

Fig 5

crCD40L is detected by mouse CD40L.

crCD40L was expressed in vaccinia virus and purified from infected BHK21 cell lysate on a nickel column. The purified protein was detected by ELISA using a mouse antibody against CD40L in a concentration gradient dependent manner. The avidity of the mouse CD40L antibody to the cotton rat CD40L protein was evaluated in the presence of 6M urea. The difference between the untreated and 6M urea treated for each group was calculated using students t-test ***p<0.001, ****p<0.0001 (n = 2). Data shown is a representative experiment of three separate experiments where two (n = 2) technical replicates are conducted in each experiment. The no-coating and no-primary antibody negative controls gave average OD values of 0.56 and 0.107 respectively.

Fig 5