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Metabolic survey of Botryococcus braunii: Impact of the physiological state on product formation

Fig 2

The overall intracellular metabolome profile of Botryococcus braunii CCAP 807/2, containing all identified metabolites during the proposed growth stages, referred as Phases II (linear phase), III (stationary phase) and IV (decline phase).

a. Non-targeted metabolome profile of primary metabolites showing the comparison of relative abundance level of metabolites, divided into three different categories based on the related metabolic pathways, thus (i) glycolysis intermediates, sugars and sugar alcohols, (ii) amino acids and other related metabolites and (iii) citric acid cycle intermediates, terpenoids, steroids and vitamins. b. Intracellular pigments with relative abundances of (i) chlorophylls and (ii) carotenoids. c. Gravimetrically determined total lipid content, containing polar (P lipids) and non-polar lipid (N-P lipids) and expressed as percentage of dry biomass weight. d. Polar lipid fraction with relative abundance level of fatty acids. e. Non-polar lipid fraction with relative abundance levels of (i) fatty acid and (ii) hydrocarbons. f. Comparison of hydrocarbons and fatty acids derived from the total non-polar lipid fraction on the basis of the relative abundance levels, considering Phase II as 100%. Metabolites were identified by ‘a’ comparison with the NIST 05 library and Golm Metabolome Database (Lib) and verified with purified standards; ‘b’ only via above mentioned databases with RSI value above 750. ‘c’ marks the identified hydrocarbons via mass spectra of GC-MS and available literature [10,17]; ‘#’ not detected. Error bars represent standard deviation (SD). Asterisks represent p-values as determined via Student’s t-test (* = < 0.05, ** = < 0.01).

Fig 2

doi: https://doi.org/10.1371/journal.pone.0198976.g002