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A disconnect between precursor frequency, expansion potential, and site-specific CD4+ T cell responses in aged mice

Fig 4

Reduced numbers of WNV-specific CD4+ T cells are present in the brain of old WNV infected mice compared to adult.

Adult and old C57BL/6 mice were infected with 1000 PFU 385–99 WNV, sc (shoulder blades). Brain and spleens were harvested on day 10 post-infection and lymphocytes were analyzed by flow cytometry. (A) Representative gating strategy for CD4+ tetramer+ T cells via forward and side scatter, live, CD3+ T cells, CD8α- CD4+ T cells and E641:I-Ab+ CD4+ T cells harvested from brain on day 10 post-WNV infection. Absolute numbers of (B) CD3+ CD4+ T cells and (C) E641:I-Ab+ CD4+ T cells and (D) Foxp3+ Tregs in the brain are shown as labeled. Results are representative of one of three experiments. Each dot represents a single mouse (*p<0.05; Mann-Whitney).

Fig 4

doi: https://doi.org/10.1371/journal.pone.0198354.g004