Secretion of autoimmune antibodies in the human subcutaneous adipose tissue
Adipocytes (AD), SVF and PBMC (blood) were sonicated for cell disruption in the presence of TRIzol to separate the soluble fraction (used for RNA isolation) from lipids and cell debris. AD and SVF were from the same obese individuals. PBMC (blood) were from obese individuals age-, gender- and BMI-matched. Results show qPCR values (2-ΔΔCt) of TNF-α and IL-6 RNA expression.