T cell mediated immunity after combination therapy with intralesional PV-10 and blockade of the PD-1/PD-L1 pathway in a murine melanoma model
Mice received 3x105 M05 tumor cells SC on bilateral flanks on Day 0. On Day 7, right flank tumors were injected with 50 μl PV-10 or PBS IL. Mice received 300 μg anti-PD-1 and NrIgG antibodies beginning on Day 8 and continuing 2x/week until mice had reached endpoint. Tumor growth was measured in (left panel) tumors treated with IL PBS or PV-10 and (right panel) untreated, bystander tumors. Each line represents the growth of tumor in a single mouse (n = 5–6 mice per treatment group).