Testing therapeutics in cell-based assays: Factors that influence the apparent potency of drugs
The antiviral activity of toremifene citrate was evaluated at an MOI of 0.5 (EBOV/Mak) with a 48 hpi endpoint in Vero E6, Huh 7 and MDM cells using the CELIA. (A) The signal-to-noise ratio and Z' factor were determined in multiple independent experiments. (B) EC50s were determined. Data were collected from 4 to over 10 experiments and the median value of all experiments was determined. Ordinary one-way ANOVA following Turkey’s post Multiple Comparison in Graphpad Prism 7.0 were performed to compare the differences of signal-to-noise ratio, Z' factor and EC 50S among three cell lines. Abbreviation: EBOV, Ebola virus; EC50, half maximal effective concentration; Mak, Makona; MDM, monocyte-derived macrophages.