Testing therapeutics in cell-based assays: Factors that influence the apparent potency of drugs
(A) Huh 7 cells were infected at an MOI of 1, and antiviral activity of toremifene citrate was evaluated at indicated time points. (B) Huh 7 cells were infected at indicated MOIs and antiviral activity of toremifene citrate was evaluated at 72 hpi. (C, D) EC50s with corresponding assay endpoints or MOIs are shown for comparison. The experiment was performed twice using the fluorescent assay. Representative graphs are shown. Abbreviations:EC50, half maximal effective concentration; hpi, hours post-inoculation; MOI, multiplicity of infection.