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Testing therapeutics in cell-based assays: Factors that influence the apparent potency of drugs

Fig 3

Impact of cell passaging on EBOV infection.

Vero E6 (A) and Huh 7 (B) cells were passaged and infected with EBOV/Mak at an MOI of 1 at 48 hpi, the plates were fixed, stained, and the percentage of EBOV-positive cells was determined by HCI. Each point is the mean of 3 replicate wells and represents an independent experiment. For each passage, the median value of all experiments was determined. Abbreviations: EBOV, Ebola virus; HCI, high content imaging; hpi, hours post-inoculation; Mak, Makona.

Fig 3