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Asymmetric expression level of clock genes in left vs. right nasal mucosa in humans with and without allergies and in rats: Circadian characteristics and possible contribution to nasal cycle

Fig 2

Representative panels showing the expression of PER1, PER2, CLOCK, and BMAL1 in nasal mucosa derived from the right (Rt) and the left (Lt) nasal cavity of rat which were evaluated by RT-PCR (A and B; A indicates the usage of GAPDH as housekeeping genes, B shows the EEF1A1 as housekeeping gene) and western blot (C). (D) Immunohistochemistry shows the distribution of PER1 (a, b), PER2 (c, d), CLOCK (e, f), and BMAL1 (g, h) in normal (a, c, e, and g) and allergic nasal mucosa (b, d, f, and h). Arrow head indicates superficial epithelium. Large arrow; submucosal glands. Small arrow; vascular endothelium. Scale bar indicates 50 uM.

Fig 2

doi: https://doi.org/10.1371/journal.pone.0194018.g002