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Development of improved therapeutic mesothelin-based vaccines for pancreatic cancer

Fig 2

Mesothelin protein is released into supernatants.

BHK cells (2x106 cells/well) were uninfected or infected with MVA, MVAmeso or MVAmesoA35Del viruses. Supernatants were collected from wells containing the mesothelin over-expressing Panc02 cells “labeled Meso Overexpressor” [7], uninfected BHK cells, or BHK cells infected with MVA viruses and analyzed by Western blot using a rabbit anti-mesothelin antibody and anti-rabbit IgG (Fc) AP Conjugate (Promega).

Fig 2

doi: https://doi.org/10.1371/journal.pone.0193131.g002