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Development of improved therapeutic mesothelin-based vaccines for pancreatic cancer

Fig 1

Mesothelin is expressed from MVAmeso viruses.

BHK cells (2x106 cells/well) were uninfected or infected with MVA, MVAmeso or MVAmesoA35Del. Cells were lysed and samples were loaded onto a pre-cast 4–20% gradient gel. Rabbit anti-mesothelin antibody and anti-rabbit IgG (Fc) AP Conjugate (Promega) detected mesothelin protein (arrow). HEK transected cells, previously described [7], were used as control.

Fig 1

doi: https://doi.org/10.1371/journal.pone.0193131.g001