Regulation of influenza virus replication by Wnt/β-catenin signaling
Mouse AECs were isolated and cultured for 6 days. (A) AECs were immuno-stained with the AEC type I marker T1α and the nuclear dye DAPI. Scale bar = 50 μm. (B-E) AECs were infected with influenza virus A/PR/8/34 or A/WSN/33 at an MOI of 1 for 24 h. The cells were treated with iCRT14 (12.5 μM) or vehicle control 1 h prior to infection. Viral titers in the cell culture medium were determined using the TCID50 assay (B, C). NA mRNA levels in cells were determined by real-time PCR and normalized to GAPDH. The results of 3 independent experiments are displayed as the mean ± SE. *p<0.05 vs. vehicle control at the corresponding time points (Student’s t-test).