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Development and testing of species-specific ELISA assays to measure IFN-γ and TNF-α in bottlenose dolphins (Tursiops truncatus)

Fig 2

Time course of IFN-γ (ng/mL) levels in supernatants from ConA or PHA stimulated PBMCs isolated from bottlenose dophins (Tursiops truncatus).

PBMCs isolated from Tursiops truncatus were stimulated with 1, 5, or 10 μg/mL PHA or 1 μg/mL ConA for 48 hours in culture. Supernatants were collected and run on an ELISA assay specific for bottlenose dolphin IFN-γ as described in Materials and Methods. Samples were collected monthly over an 11-month period from four bottlenose dolphins (Animals A, B, C, and D). Month of the study is denoted on the x-axis and month 1 corresponds to June 2014.

Fig 2