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Identification of a novel linear B-cell epitope in nonstructural protein 11 of porcine reproductive and respiratory syndrome virus that are conserved in both genotypes

Fig 3

Identifying B-cell epitope of 3F9.

(A) Four overlapped fragments covered the nsp1140-223aa were expressed by using vector PEGFP-N1, and then the mAb 3F9 reactivity of truncated nsp11 proteins were verified by WB. (B) The mAb 3F9 reactivity of nine overlapped fragment covered nsp1184-133aa were further tested by WB. (C) Eight overlapped fragments covered the nsp11109-118aa were further tested by WB. (D) The identified core unit was expressed in PEGFP-N1, for testing its mAb 3F9 reactivity by WB, with the nsp1140-223aa as positive control.

Fig 3

doi: https://doi.org/10.1371/journal.pone.0188946.g003