Identification of a novel linear B-cell epitope in nonstructural protein 11 of porcine reproductive and respiratory syndrome virus that are conserved in both genotypes
Fig 1
Expression and purification of His-tagged nsp1140-223aa protein.
(A) After culture and IPTG induction, the plasmid pET28a-nsp11 transformed E coli cells were collected. The thallus, as well as supernatant and precipitation parts post lysing, were submitted to SDS-PAGE for detecting the nsp11 expression. (B) The purity and concentration of purified nsp1140-223aa protein was analyzed in SDS-PAGE.