Oral immunization of a non-recombinant Lactococcus lactis surface displaying influenza hemagglutinin 1 (HA1) induces mucosal immunity in mice
Fig 1
Binding optimization of HA1/L/AcmA recombinant protein to L. lactis.
(A) The percentage of L. lactis surface displaying HA1/L/AcmA recombinant protein after incubation with different amounts of HA1/L/AcmA recombinant protein. (B) The percentage of L. lactis surface displaying HA1/L/AcmA recombinant protein after incubation with HA1/L/AcmA recombinant protein for 1 h, 2 h, 3 h, and 4 h, respectively. (C) The percentage and mean fluorescence intensity (MFI) value of L. lactis surface displaying HA1/L/AcmA recombinant protein after incubation with HA1/L/AcmA recombinant protein in GM17 and PBS, respectively. The data represents mean ± standard deviation. Asterisk indicates statistically significant differences between groups (**P<0.01).