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A simpler and more cost-effective peptide biosynthetic method using the truncated GST as carrier for epitope mapping

Fig 3

SDS-PAGE analysis of expressed 8/18mer peptide fusion proteins for screening r-clones.

Lane 1/8, prestained protein markers; lane 2, GST188 protein expressed by pXXGST-2; lanes 3–4, 8mer peptides P115-116 of huZP4; lanes 5–6, 18mer peptides P31-32 of huZP4; and lane 7, negative control for self-ligation of pXXGST-3. Arrows show the control GST188 or each 8/18mer peptide fusion proteins.

Fig 3

doi: https://doi.org/10.1371/journal.pone.0186097.g003