A simpler and more cost-effective peptide biosynthetic method using the truncated GST as carrier for epitope mapping
(a, b) BSP-based design for both plus and minus strands of huZP4 gene fragments encoding 18/8mer peptides, and (c) sequencing result of plus strand inserted fragment encoding P31. Pink color in all boxes indicates the paired bp in each double strand and overlapping regions of 10/7 aa between designed P31-32 and P115-116. Other colors indicate various paired bp in four of double strands. The underlines in Fig 2c indicate BamH I and Sal I restriction sites.