Expression and regulation of the neutral amino acid transporter B0AT1 in rat small intestine
Animals fed NP, HP or AA diet for 7 days were euthanized 3 h after light-onset (ZT3) or offset (ZT15). A: The transport of L-isoleucine into everted rings of the proximal and distal jejunum was measured in the presence and absence of sodium and sodium-dependent uptake was calculated. Data represent mean values of 3 intestinal ring uptakes of 9 different animals tested in 3 independent experiments. B-D: Western blotting experiments with antibodies directed against B0AT1, ACE2, CD13 and β-actin were performed in bbmv of the proximal and distal jejunum (same preparations as in Fig 1). The intensity of the immunoreactive bands was quantified, standardized to β-actin and normalized to NP diet. Data represent mean values of 9 different animals. All values are expressed as means ± SEM, one-way ANOVA and Bonferroni’s post test was performed for each time point; * P < 0.05, ** P < 0.01, *** P < 0.001.