Identification of factors involved in dimorphism and pathogenicity of Zymoseptoria tritici
(A) Map of T-DNA construct used for genomic integration, containing HPT cassette (promoter+ORF+NOS terminator) flanked by left- and right-borders. HindIII restriction enzyme was used for the restriction of genomic DNA derived from the random mutants prior to the following Southern Blot analysis. The red bar indicates the location of probe used for hybridization. (B) Southern Blot analysis of 40 representative random transformants of Z. tritici. Genomic DNA of respective strains was restricted with HindIII. Black asterisks represent most likely a single T-DNA integration event; two or more hybridization signals indicate that most likely two or multiple T-DNA integrations in the recipient genome at different sites might have occurred. M: DNA Molecular Weight Marker VII, DIG-labeled (Roche Applied Biosciences). (C) Map of pCAMB-HPT (SalI)-vector used for ATMT. (D) Pie chart showing the relative percentage of occurred T-DNA integration events in the generated random mutants (the estimated percentage refers to 100 randomly selected transformants).