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Effects of oxidative and thermal stresses on stress granule formation in human induced pluripotent stem cells

Fig 5

Pluripotent RNA binding proteins, L1TD1, LIN28A, and DPPA5 are components of SGs.

Representative fluorescence microscopy images showing non-treated hiPSCs (No treat and 37°C) and cells treated with sodium arsenite (125μM) or subjected to heat shock (42°C) stained with the SG marker (G3BP (red)) and different pluripotent markers (A) L1TD1, (B) LIN28A, or (C) DPPA5 (green). At the right of each panel, insets show magnified views of SGs in individual and merged channels (yellow). White arrows indicate SGs and yellow arrows indicate L1TD1 aggregates. Scale bar indicates 20μm.

Fig 5

doi: https://doi.org/10.1371/journal.pone.0182059.g005