Effects of oxidative and thermal stresses on stress granule formation in human induced pluripotent stem cells
(A) RT-PCR on mRNA extracted from non-treated hiPSCs (No treat and 37°C) or cells subjected to sodium arsenite, H2O2, and heat shock, to detect pluripotent genes, OCT4, SOX2, NANOG, KLF4, L1TD1, and LIN28A. GAPDH is used as a loading control. (B) Representative Immuno-blots illustrating the protein levels of OCT4, SOX2, NANOG, KLF4, L1TD1, and LIN28A. (C) Bar graphs represent the levels (normalized with β-actin loading controls) of the tested proteins. Asterisks indicate statistical significance p values; *p<0.05, **p<0.001 and ***p<0.0001. The values are expressed in mean ± SEM from 3 experiments.