Effects of oxidative and thermal stresses on stress granule formation in human induced pluripotent stem cells
(A) Representative fluorescence microscopy images showing non-treated hiPSCs (No treat and 37°C), cells treated with 125μM sodium arsenite and H2O2 (250μM and 2mM) or subjected to heat shock (42°C) stained with the robust SG marker (G3BP (green)). Nucleus is stained in blue (Hoechst). Insets show magnified views of SGs. White arrows indicate SGs. Scale bar indicates 50μm. (B) Percentage of hiPSCs with G3BP positive SGs after 1h treatment with the indicated concentrations of sodium arsenite, or concentration range of H2O2 (25, 50, 100, 250, 500μM, 1mM, and 2 mM) or subjected to heat shock at the indicated temperatures. The average percentage of cells with SGs is shown. Error bars indicate the ± standard deviation from 3 independent experiments.