cAMP-dependent activation of protein kinase A attenuates respiratory syncytial virus-induced human airway epithelial barrier disruption
Confluent airway epithelial cell monolayers were treated for 24 h with polyI:C (5 μg/ml) in the presence or either vehicle, forskolin (20 μM), or cAMP analogs (100 μM). (A) Barrier permeability was determined by measuring transepithelial dextran flux. Data is presented as mean ± SEM (n = 3); ***, P< 0.001. (B) The structure of the epithelial AJC was determined by immunofluorescence labeling and confocal microscopy of different TJ and AJ proteins. Note the disruption in normal TJ and AJ labeling pattern after polyI:C exposure (short arrows), and preservation of normal junction labeling in polyI:C-treated cells in the presence of either forskolin or 8-Bromo-cAMP. Scale bar, 40 μm. Image is representative of at least 3 independent experiments.