cAMP-dependent activation of protein kinase A attenuates respiratory syncytial virus-induced human airway epithelial barrier disruption
(A) Polarized epithelial cells were infected with RSV or rgRSV (MOI, 0.5), for 48 h in the presence of the vehicle, forskolin, or cAMP analogs. RSV A2 F mRNA in epithelial cell monolayers was determined by RT-PCR analysis. (B, C) GFP-positive rgRSV-infected cells were visualized and counted by immunofluorescence microscopy. Each image is representative of at least 3 independent experiments. Data is presented as mean ± SEM (n = 3) **, P< 0.01 and ***, P< 0.001 as compared to RSV-infected vehicle-treated cells.