Infectious bronchitis corona virus establishes productive infection in avian macrophages interfering with selected antimicrobial functions
Fig 3
Quantification of IBV M41 and Conn A5968 antigens in macrophages in chickens.
Six days old chickens were infected intra-tracheally as indicated in the Fig 1 legend and trachea and lungs were sampled at 4 dpi for double immunostaining of macrophages and IBV antigens. The sections were stained for both macrophages and IBV antigen as has been described in the materials and methods and a confocal microscope was used to obtain representative images. Macrophages were visualized with Dylight 550® (red) and IBV antigens were visualized with Dylight 488® (green). Statistical analysis for identifying group differences was done based on Student’s t test. Nuclei were stained with DAPI (blue). IBV infected macrophages are shown with arrow heads in the merged picture. Both isotype and uninfected controls are shown.