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Reference gene selection for the shell gland of laying hens in response to time-points of eggshell formation and nicarbazin

Fig 4

Comparison of the relative expression stability of 10 candidate reference genes analysed by geNorm, NormFinder and BestKeeper.

The geNorm M and SD values of 10 reference genes were graphed to assess the ranking pattern of the genes assigned by three different software. A). Four different time-points (2, 5, 15, 23.5 hr) post-oviposition in the birds at age of 36–37 weeks. B). Groups treated with or without nicarbazin at three different time-points (5, 15, 23.5 hr) post-oviposition of birds at 42–45 weeks. C). Three different time-points (5, 15, 23.5 hr) post-oviposition times of the birds without nicarbazin treatment at age of 42–45 weeks. D). Three different time-points (5, 15, 23.5 hr) post-oviposition of the birds with nicarbazin treatment at age of 42–45 weeks.

Fig 4

doi: https://doi.org/10.1371/journal.pone.0180432.g004