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PTH[1-34] improves the effects of core decompression in early-stage steroid-associated osteonecrosis model by enhancing bone repair and revascularization

Fig 3

MRI images and histological analysis confirmed successful establishment of the SAON model.

(A) MRI images of femurs on T1 and T2 weight from the SAON group (n = 4) and the Sham group before and 2 weeks after steroid administration, exhibited diffused and decreased SI (red arrows) in T1W. Focal hypointense lesion (green arrows) was surrounded by diffuse hyperintense area in T2W at week 2, but no abnormal signal was found at week 0. MRI images of femurs in T1 and T2 weight from the sham group (n = 4) were obtained at week 0 and week 2 after vehicle injection, and displayed no abnormal SI. (B) Histological analyses of distal femurs from each group (n = 4). H&E staining after steroid-induced osteonecrosis showed necrotic mass, enlarged fat cells and massive empty osteocyte lacunae (black arrows) in the SAON group. The ER on T2 weight MR images (C) and the area of abnormal SI (D) were measured for the quantification of MR images. The percentage of empty osteocyte lacunae (E) and the mean fat cell diameter (F) were measured. Data are presented as mean ± SD, and error bars in the figure denote SD, **p<0.01,*p<0.05.

Fig 3

doi: https://doi.org/10.1371/journal.pone.0178781.g003