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Establishment a real-time reverse transcription PCR based on host biomarkers for the detection of the subclinical cases of Mycobacterium avium subsp. paratuberculosis

Fig 2

Amplification plots of the biomarker genes in the real-time PCR.

Real-time PCR was conducted with PCR product that serially diluted 10-fold from 109 to 102 copy numbers. The emission of fluorescence was measured at each cycle numbers and negative control sample with no template DNA showed no increasing of fluorescence.

Fig 2