Development of the full-length cDNA clones of two porcine epidemic diarrhea disease virus isolates with different virulence
(A) The virus-induced CPE at 24 h post infection (MOI = 0.01). (B) Indirect immunofluorescence staining of each virus with mouse monoclonal antibody 4E3 to PEDV N protein. (C) Detection of the genetic marker by sequencing of the recombinant viruses. (D) Multi-step growth curve of WT and recombinant viruses on Vero cells at an MOI of 0.01. (E) Comparison of plaque sizes and morphology of WT PEDV and the infectious clone-derived viruses on Vero cells at 4 days post infection. Asterisk (*) indicates a significant difference between rBJ2011C and rCHM2013 (*P<0.05; **P<0.01; ***P<0.001).