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Development of the full-length cDNA clones of two porcine epidemic diarrhea disease virus isolates with different virulence

Fig 2

Construction of the full-length clones of PEDV strains BJ2011C and CHM2013 in the BAC system.

The cDNA segments of PEDV BJ2011C (A) and CHM2013 (B) were cloned into the BAC plasmid to assemble the full-length cDNA clones. The PEDV cDNA genome was set under the control of the CMV promoter and followed by a HDV ribozyme sequence and a BGH polyadenylation signal. The restriction enzyme sites and the divided gene segments were also indicated that allowed the sequential assembly of a full-length cDNA clone in the BAC plasmid.

Fig 2

doi: https://doi.org/10.1371/journal.pone.0173998.g002