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The 9aaTAD Is Exclusive Activation Domain in Gal4

Fig 2

Artificial peptides in the original Gal4 constructs activated transcription.

To distinguish natural and artificial activity, a sets of LexA-Gal4 hybrid constructs were generated with and without artificial peptides presented in original constructs, an unintended consequence of cloning [24]. The LexA-Gal4 hybrid constructs assayed in L40 strain for transactivation activity are shown. The average value of the β-galactosidase activities from three independent experiments is presented as a percentage of the reference with standard deviation (means and plusmn; SD; n = 3). We standardized all results to previously reported Gal4 construct HaY including merely the activation domain 9aaTAD with the activity set to 100% [34]. The protein sequences are partially given by amino acid single letter code. Single dot means end of protein sequence, tree points mean continuing of the sequence, which is not more shown. The regions of Gal4 protein in the constructs are noted and graphically presented.

Fig 2

doi: https://doi.org/10.1371/journal.pone.0169261.g002