The 9aaTAD Is Exclusive Activation Domain in Gal4
The Gal4—LexA hybrid constructs (BTM116 backbone) were assayed in L40 strain for activation of transcription. To distinguish activity of the N- and C-terminal activation domains in the Gal4 protein, a set of the Gal4 constructs were generated. The average value of the β-galactosidase activities from three independent experiments is presented as a percentage of the reference with standard deviation (means and plusmn; SD; n = 3). We standardized all results to previously reported Gal4 construct HaY including merely the activation domain 9aaTAD with the activity set to 100% . The LexA is E.coli DNA binding domain and the Gal4 DBD is S.cerevisiae Gal4 DNA binding domain, both generally used for the generation of fusion hybrids. The regions of Gal4 protein in the constructs are noted and graphically presented. Single dot means end of protein sequence, tree points mean continuing of the sequence, which is no more shown.