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High-Throughput Analysis of Global DNA Methylation Using Methyl-Sensitive Digestion

Fig 3

Application of the MSFP assay to human genomic DNA.

(A) Digestion pattern of THP-1 genomic DNA digested with MspI, HpaII, or a combination of HpaII and HpyCH4IV (HH). The digested DNA was separated by agarose gel electrophoresis. UD; undigested. (B) Standard curves for THP-1 DNA in the MSFP assay. Data represent medians and ranges of three independent experiments performed in technical duplicates or triplicates. The amounts of digested DNA applied per well are indicated on the x-axis.

Fig 3

doi: https://doi.org/10.1371/journal.pone.0163184.g003