Multiplex Real-Time PCR Assays that Measure the Abundance of Extremely Rare Mutations Associated with Cancer
Fig 4
SuperSelective primer elements that affect selectivity.
The linearity of the relationship between the threshold cycle and the logarithm of the initial number of mutant templates present in reactions containing 106 wild-type templates and different amounts of mutant template was determined for PCR assays initiated with different SuperSelective primer designs. (A) PCR reactions initiated with EGFR L858R SuperSelective primers possessing foot sequences of different length. (B) PCR reactions initiated with EGFR L858R SuperSelective primers that form symmetrical bubbles of different circumference.