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Multiplex Real-Time PCR Assays that Measure the Abundance of Extremely Rare Mutations Associated with Cancer

Fig 3

Primers utilized in monoplex PCR assays.

The bridge sequence within each SuperSelective primer is shown in blue, and the interrogating nucleotide in each foot sequence is shown in red. The EGFR L858R primers are arranged into groups that reflect their use in comparative experiments. The BRAF V600E target sequence is 69 base pairs long; the EGFR L858R target sequences are between 79 and 87 base pairs long, depending on the SuperSelective primer that is used; and the EGFR T790M target sequence is 68 base pairs long.

Fig 3