Enhanced Rice Blast Resistance by CRISPR/Cas9-Targeted Mutagenesis of the ERF Transcription Factor Gene OsERF922
Fig 3
Identification of blast resistance in homozygous mutant rice lines.
(A) Nucleotide sequences of the target site in the 6 homozygous T2 mutant rice lines used for pathogen inoculation. The recovered mutated alleles are shown below the wild-type sequence. The target site nucleotides are indicated using black capital letters and black dashes. The PAM site nucleotides are underlined. Red dashes indicate the deleted nucleotides. Red capital letters indicate the inserted nucleotides. The numbers on the right indicate the type of mutation and the number of nucleotides involved. “−” and “+” indicate the deletion and insertion of the indicated number of nucleotides, respectively. (B) The blast resistance phenotypes of the mutant rice lines and wild-type plants at the seedling stage. Leaves were detached from the inoculated plants at 7 dpi for photography. The experiments were repeated three times with similar results. (C) Histograms showing the average area of lesions formed on the third leaves of 10 plants for each line. The values marked with different letters are significantly different (P < 0.01, Student’s t-test). (D) Blast resistance phenotypes of the mutant rice lines and the wild-type plants at the tillering stage. Leaves were detached from the inoculated leaves of plants at 7 dpi for photography. The experiments were repeated three times with similar results. (E) Histograms showing the average length of lesions formed on the inoculated leaves of five tillerings for each line. The values marked with different letters are significantly different (P < 0.01, Student’s t-test).