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Fluorescence Polarization Screening Assays for Small Molecule Allosteric Modulators of ABL Kinase Function

Fig 2

Recombinant ABL Ncap-SH3-SH2-linker (N32L) proteins.

Wild type ABL N32L protein and the corresponding high-affinity linker (HAL) and W118A mutants were expressed in E. coli using the pET system [40] and purified by immobilized metal affinity chromatography. Protein purity and mass were verified by SDS polyacrylamide gel electrophoresis (A) and mass spectrometry (B).

Fig 2

doi: https://doi.org/10.1371/journal.pone.0133590.g002