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Comparative Analysis of Stress Induced Gene Expression in Caenorhabditis elegans following Exposure to Environmental and Lab Reconstituted Complex Metal Mixture

Fig 5

Effect of metals exposure on nuclear hormone receptors and developmental genes in C. elegans.

C. elegans treated with the metal cocktail (MC) or waters from Lake Hornträsket (H) and Vombäcken stream at Björnås (B) and the exit to Vindelälven (V) were analyzed for the expression of genes associated with hormone receptors (fshr-1, nhr-8 and nhr-14) and development (vit-2, daf-12, and apl-1) were tested using qRT-PCR. One way analysis of variance (ANOVA) followed by Tukey’s post-test was used for multiple group comparison where * refers to p<0.01 as compared to control whereas # represents significant differences in between the sites H, B and V. The dotted line represents the expression level normalized to that of the control (K-medium prepared with Milli-Q water).

Fig 5

doi: https://doi.org/10.1371/journal.pone.0132896.g005