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Comparative Analysis of Stress Induced Gene Expression in Caenorhabditis elegans following Exposure to Environmental and Lab Reconstituted Complex Metal Mixture

Fig 3

Exposure of C. elegans to metals affects the expression of oxidative stress response genes.

Synchronized C. elegans treated with the metal cocktail (MC) or waters from Lake Hornträsket (H) and Vormbäcken stream at Björkås (B) and the exit to Vindelälven (V) were analyzed for the expression of gst-4, sod-1, cat-2, cyp-35A2, daf-2, nnt-1, kel-8 and gcs-1 using qRT-PCR. One way analysis of variance (ANOVA) followed by Tukey’s post-test was used for multiple group comparison where * refers to p<0.01 as compared to control and # represents significant differences in between sites H, B and V. The dotted line represents the expression level normalized to that of the control (K-medium prepared with Milli-Q water).

Fig 3