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ITS2 Secondary Structure Improves Discrimination between Medicinal “Mu Tong” Species when Using DNA Barcoding

Fig 1

The predicted ITS2 secondary structure of the “Mu tong” taxa.

(a) Consensus secondary structure model of the ITS2 region based on 30 sequences covering four families (Actinidiaceae, Aristolochiaceae, Lardizabalaceae and Ranunculaceae). The four helices, each with a stem–loop, are labeled I–IV. Compatible base pairs are colored and the degree of conservation over the whole alignment is indicated with different degrees of color saturation. (b) One of the example secondary structures of Akebia quinata. (c) The position of the strictly conserved base pair sites (highlight in blue circle) found in the 70% consensus ITS2 secondary structure model.

Fig 1

doi: https://doi.org/10.1371/journal.pone.0131185.g001