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Characterization of Cereulide Synthetase, a Toxin-Producing Macromolecular Machine

Fig 7

NADPH affinity and turnover in the KR domains in CesA1 and CesB1.

(A,B) Fluorescence intensity enhancement is plotted against protein concentration using a fixed concentration of NADPH (2.5 μM); λexc = 340nm and λemm = 445nm for CesA1 and CesB1. Curves were fitted to a Morrison equation that includes terms for enhancement of NADPH fluorescence upon binding. (C) Enzymatic NADPH consumption as reported by fluorescence intensity (λexc = 340nm and λemm = 445nm).

Fig 7

doi: https://doi.org/10.1371/journal.pone.0128569.g007