A Recombinant Fungal Lectin for Labeling Truncated Glycans on Human Cancer Cells
Fig 4
PVL binding of tumor cell lines.
A. Flow cytometry histograms show rPVL-Alexa 488 binding to a lung immortalized cell line (HBEC-3KT), two lung tumor cell lines (H358 and A549) as well as on a breast tumor cell line (MCF-7). The x axis indicates fluorescence intensity. The y axis indicates cell number. Black line: untreated control cells; blue line rPVL-Alexa 488 5 μg ml-1 for 30 mn; red line: rPVL-alexa 488 5 μg ml-1 in the presence of GlcNAc 100 mM; green line: rPVL-Alexa 488 5 μg ml-1 after sialidase pretreatment. B. Microscopy images of A549 NSCLC cells treated for 30 min at 37°C with 5 μg ml-1 rPVL labeled with Alexa 488 in the presence or absence of 100 mM GlcNAc. Green channel shows rPVL-Alexa 488, blue channel shows nuclei labeled with DAPI staining.