Efficient Differentiation of Steroidogenic and Germ-Like Cells from Epigenetically-Related iPSCs Derived from Ovarian Granulosa Cells
Fig 4
mGriPSC-EBs differentiate to express ovarian and primordial oocyte markers.
Differentiating mGriPSC-EBs express ovarian markers AMHR (A), FSHR (B), Cyp19a (C), Inha (D), and ER (E) (phase contrast; F) at higher frequencies than G4- and mFiPSC-EB (G). Expression of germ cell markers Mvh (H), Dazl (I), Gdf9 (J), Boule (K), and Zp1 (L) (phase contrast; M) are also increased in mGriPSC-EBs (N). Two-tailed t-test, *P < 0.05. Scale bars: A-F 10 μm; H-M 20 μm.