Skip to main content
Advertisement
Browse Subject Areas
?

Click through the PLOS taxonomy to find articles in your field.

For more information about PLOS Subject Areas, click here.

< Back to Article

Efficient Differentiation of Steroidogenic and Germ-Like Cells from Epigenetically-Related iPSCs Derived from Ovarian Granulosa Cells

Fig 3

mGriPSC-EBs preferentially synthesize estradiol.

Mouse GCs (A) infected with pluripotency genes generate iPSC colonies. Arrow indicates primary granulosa cells. mGriPSC-EBs, when allowed to attach, synthesize estradiol (B) at concentrations 5 to 12 times those of G4-EBs and mFiPSC-EBs. Two-tailed t-test, *P<0.001, different from other two cell lines at same time point. G4-EBs primarily secreted progesterone (C) while mFiPSC-EBs produced negligible amounts of either. Two-tailed t-test, *P<0.005, different from other two cell lines at same time point. Scale bar: A 100 μm.

Fig 3

doi: https://doi.org/10.1371/journal.pone.0119275.g003