ABL Tyrosine Kinase Inhibition Variable Effects on the Invasive Properties of Different Triple Negative Breast Cancer Cell Lines
(A, B) Effect of nilotinib on BT-549 and MDA-MB 231 cell area. The area of 100 BT-549 (A) and MDA-MB 231 (B) cells plated on Oregon Green 488 gelatin was measured after incubation with DMSO or 100nM nilotinib and F-actin staining. Results are the mean ± SEM relative to control. *p<0.05 compared to DMSO-treated cells. Scale bar: 20μm. (C, D) Degradation area of 100 BT-549 (C) and MDA-MB 231 (D) cells incubated with DMSO, 100nM nilotinib, 2μg/ml C3, 100μM NSC23766 or 25μM ITX3 for 3h were measured as described in Fig. 1A. Results are the mean ± SEM, *p<0.05 compared to DMSO-treated cells.